An integrated strategy involving high‐throughput sequencing to characterize an unknown GM wheat event in Canada
An integrated strategy involving high‐throughput sequencing to characterize an unknown GM wheat event in Canada
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Full item details
- dc.contributor.author
- Gagnon, Marie‐Claude
- Duceppe, Marc‐Olivier
- Colville, Adam
- Pope, Louise
- Côté, Marie‐José
- Ogunremi, Dele
- dc.date.accepted
- 2023-11-04
- dc.date.accessioned
- 2024-08-29T15:18:52Z
- dc.date.available
- 2024-08-29T15:18:52Z
- dc.date.issued
- 2023-12-05
- dc.date.submitted
- 2023-05-17
- dc.description.abstract - en
- Glyphosate-resistant wheat plants were discovered in southern Alberta in 2017, representing an unauthorized GM release in Canada. The Canadian Food Inspection Agency undertook a series of experiments to characterize and identify this unknown GM wheat, as well as to develop and validate construct-specific and event-specific qPCR assays. Results of PCR-based assays and Sanger sequencing indicated the presence of CaMV 35S promoter (p35S), Rice Actin 1 intron (RactInt1), CP4-EPSPS gene and nopaline synthase terminator (tNOS) elements in the unknown GM wheat. Genome walking and bead capture strategies, combined with high-throughput sequencing, were used to identify the 5′ and 3′ wheat junctions and the subsequent mapping of the insert to chromosome 3B of the wheat genome. A probable transformation vector, pMON25497, was recognized, and further testing identified the unknown GM wheat as MON71200 event, one of two events obtained with the pMON25497 vector. The two construct-specific assays targeted the junctions of the RactInt1 and the CP4-EPSPS elements and the CP4-EPSPS and tNOS elements, while the event-specific assay was located at the 3′ junction into the wheat genome. Both construct-specific and event-specific assays had limits of detection of 0.10% of MON71200 in a seed pool. As expected, the two construct-specific assays cross-reacted with other wheat and corn events containing the same elements in the same order. No cross-reactivity was observed for the event-specific assay. The integrated strategy employed in this study can serve as a model for other cases when facing similar challenges involving unknown GM events.
- dc.identifier.citation
- Gagnon, M., Duceppe, M., Colville, A., Pope, L., Côté, M., & Ogunremi, D. (2023). An integrated strategy involving high‐throughput sequencing to characterize an unknown GM wheat event in Canada. Plant Biotechnology Journal, 22(4), 904–914. https://doi.org/10.1111/pbi.14232
- dc.identifier.doi
- https://doi.org/10.1111/pbi.14232
- dc.identifier.issn
- 1467-7652
- dc.identifier.uri
- https://science-ouverte.canada.ca/handle/123456789/2901
- dc.language.iso
- en
- dc.publisher
- John Wiley & Sons, Inc
- dc.rights - en
- Creative Commons Attribution 4.0 International (CC BY 4.0)
- dc.rights - fr
- Creative Commons Attribution 4.0 International (CC BY 4.0)
- dc.rights.openaccesslevel - en
- Gold
- dc.rights.openaccesslevel - fr
- Or
- dc.rights.uri - en
- https://creativecommons.org/licenses/by/4.0/
- dc.rights.uri - fr
- https://creativecommons.org/licenses/by/4.0/deed.fr
- dc.subject - en
- Agriculture
- Science and technology
- dc.subject - fr
- Agriculture
- Sciences et technologie
- dc.subject.en - en
- Agriculture
- Science and technology
- dc.subject.fr - fr
- Agriculture
- Sciences et technologie
- dc.title - en
- An integrated strategy involving high‐throughput sequencing to characterize an unknown GM wheat event in Canada
- dc.type - en
- Article
- dc.type - fr
- Article
- local.article.journalissue
- 4
- local.article.journaltitle
- Plant Biotechnology Journal
- local.article.journalvolume
- 22
- local.pagination
- 904-914
- local.peerreview - en
- Yes
- local.peerreview - fr
- Oui
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