Differential expression of genes associated with non-target site resistance in Poa annua with target site resistance to acetolactate synthase inhibitors

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creativework.keywords - en
RNA sequencing
transcriptomics
non-target site resistance
turfgrasses
creativework.keywords - fr
séquençage de l'ARN
transcriptomiques
résistance des sites non ciblés
pelouses
dc.contributor.author
Laforest, M.
Soufiane, B.
Patterson, E. L.
Vargas, J. J.
Boggess, S. L.
Houston, L. C.
Trigiano, R. C.
Brosnan, J. T.
dc.date.accessioned
2023-04-20T15:37:38Z
dc.date.available
2023-04-20T15:37:38Z
dc.date.issued
2021-07-12
dc.description.abstract - en
Abstract BACKGROUND Poa annua is a pervasive grassy, self-pollinating, weed that has evolved resistance to 10 different herbicide modes-of-action, third most of all weed species. We investigated constitutive overexpression of genes associated with non-target site resistance (NTSR) in POAAN-R3 and the response of those genes when treated with trifloxysulfuron despite the biotype having a known target site mutation in acetolactate synthase (ALS). RESULTS Despite having an ALS target site mutation, POAAN-R3 still had a transcriptomic response to herbicide application that differed from a susceptible biotype. We observed differential expression of genes associated with transmembrane transport and oxidation–reduction activities, with differences being most pronounced prior to herbicide treatment. CONCLUSIONS In the P. annua biotype we studied with confirmed target site resistance to ALS inhibitors, we also observed constitutive expression of genes regulating transmembrane transport, as well as differential expression of genes associated with oxidative stress after treatment with trifloxysulfuron. This accumulation of mechanisms, in addition to the manifestation of target site resistance, could potentially increase the chance of survival when plants are challenged by different modes of action.
dc.identifier.citation
Laforest, J., Soufiane, B., Patterson, E. L., Vargas, J. J., Boggess, S. L., Houston, L. C., Trigiano, R. C., & Brosnan, J. T. (2021). Differential expression of genes associated with non-target site resistance in Poa annua with target site resistance to acetolactate synthase inhibitors. Pest Mangement Science, 77(11), 4993-5000. https://doi.org/10.1002/ps.6541
dc.identifier.doi
https://doi.org/10.1002/ps.6541
dc.identifier.issn
1526-4998
dc.identifier.uri
https://science-ouverte.canada.ca/handle/123456789/220
dc.language.iso
en
dc.publisher
John Wiley & Sons Ltd.
dc.rights.openaccesslevel - en
Green
dc.rights.openaccesslevel - fr
Vert
dc.subject - en
Nature and environment
dc.subject - fr
Nature et environnement
dc.subject.en - en
Nature and environment
dc.subject.fr - fr
Nature et environnement
dc.title - en
Differential expression of genes associated with non-target site resistance in Poa annua with target site resistance to acetolactate synthase inhibitors
dc.title.fosrctranslation - fr
Differential expression of genes associated with non-target site resistance in Poa annua with target site resistance to acetolactate synthase inhibitors
dc.type - en
Article
dc.type - fr
Article
local.article.journalissue
11
local.article.journaltitle
Pest Management Science
local.article.journalvolume
77
local.article.pagination
4993-5000
local.peerreview - en
Yes
local.peerreview - fr
Oui
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