Verification and use of the US-FDA BAM 19b method for detection of Cyclospora cayetanensis in a survey of fresh produce by CFIA laboratory

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creativework.keywords - en

Trichinellosis and other foodborne zoonotic pathogens

Trichinella

creativework.keywords - fr

Trichinellose et autres pathogènes zoonotiques d'origine alimentaire

Trichinella

dc.contributor.author

Lalonde, Laura

Oakley, Jenna

Fries, Patrick

dc.date.accepted

2022-02-24

dc.date.accessioned

2025-02-26T19:00:08Z

dc.date.available

2025-02-26T19:00:08Z

dc.date.issued

2022-03-04

dc.date.submitted

2022-01-28

dc.description.abstract - en

To facilitate the harmonized surveillance and investigation of cyclosporiasis outbreaks in the US and Canada, we adapted and verified the US-FDA’s BAM 19b method and employed it in a national produce survey. Performance was verified by spiking 200, 10, 5 or 0 C. cayetanensis oocysts onto berries (50 ± 5 g, n = 85) and 200, 10 or 0 oocysts onto green onions (25 ± 3 g, n = 24) and leafy greens (25 ± 1 g, n = 120) and testing these samples by the BAM method on Bio-Rad CFX96. Method robustness was assessed by aging (0 or 7 days) and freezing the produce and washes prior to testing, then implementing the method for the surveillance testing of 1759 imported leafy green, herb and berry samples. Diagnostic sensitivity was 100/44% and 93/30% for berries and leafy greens spiked with 200/10 oocysts, respectively. The diagnostic and analytical specificity were 100% for all matrices and related parasites tested. The proportion positive was unaffected (p = 0.22) by age or condition of produce (7d, fresh, frozen) or wash concentrate (3d, fresh, frozen); however, the Cq values were higher (p = 0.009) for raspberries aged 7d (37.46 ± 0.29) compared to fresh (35.36 ± 0.29). C. cayetanensis was detected in berries (two), herbs (two) and leafy greens (one), representing 0.28% of the tested survey samples. These results independently verified the reported performance characteristics and robustness of the BAM method for the detection of C. cayetanensis in a variety of matrices, including under adverse sample conditions, using a unique detection platform and demonstrating its routine diagnostic use in our Canadian Food Inspection Agency (CFIA) laboratory.

dc.identifier.citation

Lalonde, L., Oakley, J., & Fries, P. (2022). Verification and use of the US-FDA bam 19b method for detection of Cyclospora cayetanensis in a survey of fresh produce by CFIA laboratory. Microorganisms, 10(3), 559. https://doi.org/10.3390/microorganisms10030559

dc.identifier.doi

https://doi.org/10.3390/microorganisms10030559

dc.identifier.issn

2076-2607

dc.identifier.uri

https://science-ouverte.canada.ca/handle/123456789/3469

dc.language.iso

en

dc.publisher - en

MDPI

dc.rights - en

Creative Commons Attribution 4.0 International (CC BY 4.0)

dc.rights - fr

Creative Commons Attribution 4.0 International (CC BY 4.0)

dc.rights.uri - en

https://creativecommons.org/licenses/by/4.0/

dc.rights.uri - fr

https://creativecommons.org/licenses/by/4.0/deed.fr

dc.subject - en

Agriculture

Food safety

Food inspection

Fruits

Vegetables

dc.subject - fr

Agriculture

Salubrité des aliments

Inspection des aliments

Fruit

Légume

dc.subject.en - en

Agriculture

Food safety

Food inspection

Fruits

Vegetables

dc.subject.fr - fr

Agriculture

Salubrité des aliments

Inspection des aliments

Fruit

Légume

dc.title - en

Verification and use of the US-FDA BAM 19b method for detection of Cyclospora cayetanensis in a survey of fresh produce by CFIA laboratory

dc.type - en

Article

dc.type - fr

Article

local.acceptedmanuscript.articlenum

559

local.article.journalissue

3

local.article.journaltitle - en

Microorganisms

local.article.journalvolume

10

local.peerreview - en

Yes

local.peerreview - fr

Oui

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