Molecular basis of an agarose metabolic pathway acquired by a human intestinal symbiont

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DOI

https://doi.org/10.1038/s41467-018-03366-x

Langue de publication
Anglais
Date
2018-03-13
Type
Article
Auteur(s)
  • Pluvinage, Benjamin
  • Grondin, Julie M.
  • Amundsen, Carolyn
  • Klassen, Leeann
  • Moote, Paul E.
  • Xiao, Yao
  • Thomas, Dallas
  • Pudlo, Nicholas A.
  • Anele, Anuoluwapo
  • Martens, Eric C.
  • Inglis, G. Douglas
  • Uwiera, Richard E.R.
  • Boraston, Alisdair B.
  • Abbott, D. Wade
Éditeur
Nature Publishing Group

Résumé

In red algae, the most abundant principal cell wall polysaccharides are mixed galactan agars, of which agarose is a common component. While bioconversion of agarose is predominantly catalyzed by bacteria that live in the oceans, agarases have been discovered in microorganisms that inhabit diverse terrestrial ecosystems, including human intestines. Here we comprehensively define the structure–function relationship of the agarolytic pathway from the human intestinal bacterium Bacteroides uniformis (Bu) NP1. Using recombinant agarases from Bu NP1 to completely depolymerize agarose, we demonstrate that a non-agarolytic Bu strain can grow on GAL released from agarose. This relationship underscores that rare nutrient utilization by intestinal bacteria is facilitated by the acquisition of highly specific enzymes that unlock inaccessible carbohydrate resources contained within unusual polysaccharides. Intriguingly, the agarolytic pathway is differentially distributed throughout geographically distinct human microbiomes, reflecting a complex historical context for agarose consumption by human beings.

Sujet

  • Bactérie,
  • Être humain

Mots-clés

  • Bacterial proteins,
  • Bacteroides,
  • Glycosidases,
  • Intestines,
  • Recombinant proteins,
  • Sepharose,
  • Symbiosis

Droits

Creative Commons Attribution 4.0 International (CC BY 4.0)

Pagination

1-14

Évalué par les pairs

Yes

Niveau de libre accès

Or

Identifiants

ISSN
2041-1723

Article

Titre de la revue
Nature Communications
Volume de la revue
9
Numéro de l'élément
1043
Date d'acceptation
2017-11-15
Date de soumission
2017-01-27

Référence(s)

Pluvinage, B., Grondin, J. M., Amundsen, C., Klassen, L., Moote, P. E., Xiao, Y., Thomas, D., Pudlo, N. A., Anele, A., Martens, E. C., Inglis, G. D., Uwiera, R. E. R., Boraston, A. B., & Abbott, D. W. (2018). Molecular basis of an agarose metabolic pathway acquired by a human intestinal symbiont. Nature Communications, 9, Article 1043. https://doi.org/10.1038/s41467-018-03366-x

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Collection(s)

Biology

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